High-Content Analysis Monitoring Intracellular Trafficking and Replication of Mycobacterium tuberculosis Inside Host Cells

Phage recombination techniques have been instrumental within the growth of gene modification applied sciences for bacterial pathogens. In specific, the Che9 phage RecET system has been used efficiently for over 10 years for making gene knockouts and fusions in Mycobacterium tuberculosis. This “recombineering” expertise sometimes makes use of linear dsDNA substrates that comprise a drug-resistance marker flanked by (as much as) 500 base pairs of DNA homologous to the goal website.

Less usually employed in mycobacterial recombineering is the use of oligonucleotides, which require solely the motion of the RecT annealase to align oligos to ssDNA areas of the replication fork, for subsequent incorporation into the chromosome. Despite the upper frequency of such occasions relative to dsDNA-promoted recombineering, oligo-mediated adjustments typically endure from the drawback of not being selectable, thus making them more durable to isolate.

This chapter discusses steps and methodologies that improve the frequencies of discovering oligo-mediated occasions, together with the switch of single nucleotide polymorphisms (SNPs) to mycobacterial chromosomes, and the use of oligos along side the mycobacterial phage Bxb1 site-specific recombination system for the straightforward era of knockouts, insertion, and fusions, in a protocol generally known as ORBIT.

In the present examine, we adopted 839 family contacts (HHCs) of tuberculosis (TB) sufferers for two years and recognized the elements that enhanced the event of TB. Fourteen of the 17 HHCs who progressed to TB had been within the 15- to 30-year-old age group. At baseline (the “0” time level, when all of the people had been wholesome), the focus of the thyroid hormone thyroxine (T4) was decrease, and there have been elevated numbers of Tregs in PBMCs of TB progressors.

At baseline, PBMCs from TB progressors stimulated with early secretory antigenic goal 6 (ESAT-6) and 10 kDa tradition filtrate antigen (CFP-10) produced much less IL-1α. Thyroid hormones inhibited Mycobacterium tuberculosis (Mtb) progress in macrophages in an IL-1α-dependent method. Mtb-infected Thra1PV/+ (mutant thyroid hormone receptor) mice had elevated mortality and decreased IL-1α manufacturing. Our findings recommend that younger HHCs who exhibit decreased manufacturing of thyroid hormones are at excessive danger of creating energetic TB illness.

Nasopharyngeal tuberculosis is a uncommon incidence, and primarily entails the proximal half of the Eustachian tube. Mechanical obstruction of the Eustachian tube will have an effect on the stress discrepancy between the center ear and the exterior ambiance, resulting in aural fullness, unilateral listening to disturbance, and otitis media.

These situations will be ignored or mistakenly identified as ear pathology. We report a case of unilateral listening to loss and tinnitus in a 31-year-old wholesome lady that was later confirmed to be tuberculosis of the Eustachian tube. In circumstances with indicators suggesting a excessive index of suspicion, an intensive examination of the top and neck areas is very crucial. Once it has been identified, Eustachian tuberculosis will be efficiently handled with anti-tuberculosis drugs.

Hollow fiber techniques (HFSs) have been broadly utilized to check pharmacokinetic-pharmacodynamic (PK-PD) relationships in antibiotic analysis and growth. The system contains a bundle of high-density hole capillary fibers that conduct a movement of medium with or with out drug and an extra-capillary area (ECS) inoculated with the pathogen of curiosity.

The semipermeable membrane of the hole fibers permits for speedy change of small molecule medication and solutes, whereas the pathogen is restricted to the ECS. The distinctive properties of the HFS are (1) the flexibility to simulate any PK profile inside the fibers and ECS, together with plasma or site-of-disease PK profiles, (2) the flexibility to concurrently enter a number of medication with totally different half-lives, (3) the flexibility to control progress situations resembling medium composition, carbon supply, and pH, and (4) the flexibility to pattern in each compartments to be able to monitor drug concentrations and bacterial progress kinetics over time.

The system is especially suited to Mycobacterium tuberculosis analysis in a biosafety stage 3 (BSL3) atmosphere since pathogenic micro organism are sequestered in an remoted compartment. The HFS was certified by the European Medicines Agency for antituberculosis drug growth in 2015. Here, we describe the usual procedures used to check the expansion kinetics of M. tuberculosis within the HFS and the killing impact of first-line antituberculous medication utilized underneath simulated human PK situations. This animal-sparing and economical instrument will be utilized to optimize dosing schedules that reduce emergence of resistance and to prioritize drug regimens that speed up sterilization.

The utility of fluorescent proteins in bacterial analysis has lengthy been appreciated, with intensive use within the Mycobacterium tuberculosis subject. In more moderen years, a brand new era of fluorescent instruments has been developed to be used in M. tuberculosis analysis. These new fluorescent reporters exploit the immense genetic and transcriptional information now accessible, and allow the use of the micro organism as direct reporters of the native atmosphere throughout an infection, in addition to present perception into bacterial replication standing in situ.

Borrelia Afzelii Antigen
NAT41583-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Harvested bacteria are washed and detergent solubilized to produce a cytoplasmic extract enriched with lipoproteins and outer surface proteins (OSPs). The antigen is presented in phosphate buffered saline containing 1 % n-octyl-β-D-glucopyranoside.

Borrelia Garinii Antigen
NAT41584-100	The Native Antigen Company	0.1	EUR 395.28
Description: Harvested bacteria are washed and detergent solubilized to produce a cytoplasmic extract enriched with lipoproteins and outer surface proteins (OSPs). The antigen is presented in phosphate buffered saline containing 1 % n-octyl-β-D-glucopyranoside.

Borrelia Garinii Antigen
NAT41584-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Harvested bacteria are washed and detergent solubilized to produce a cytoplasmic extract enriched with lipoproteins and outer surface proteins (OSPs). The antigen is presented in phosphate buffered saline containing 1 % n-octyl-β-D-glucopyranoside.

Brucella Abortus Antigen
NAT41599-100	The Native Antigen Company	0.1	EUR 395.28
Description: Brucella abortus lysate, produced by fermentation.

Brucella Abortus Antigen
NAT41599-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Brucella abortus lysate, produced by fermentation.

Candida Albicans Antigen
NAT41601-100	The Native Antigen Company	0.1	EUR 395.28
Description: Candida albicans is grown on solid medium, washed, disrupted and subjected to an extraction process. The antigen consists of cytoplasmic and cell wall components.

Candida Albicans Antigen
NAT41601-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Candida albicans is grown on solid medium, washed, disrupted and subjected to an extraction process. The antigen consists of cytoplasmic and cell wall components.

Canine Parvovirus Antigen
NAT41594-100	The Native Antigen Company	0.1	EUR 395.28
Description: Canine Parvovirus, produced in CRFK cells.

Canine Coronavirus Antigen
NAT41590-100	The Native Antigen Company	0.1	EUR 395.28
Description: Canine Coronavirus produced in A-72 cells.

Feline Calicivirus Antigen
NAT41595-100	The Native Antigen Company	0.1	EUR 395.28
Description: Feline Calicivirus produced in FC 3TG cells.

Helicobacter Pylori Antigen
NAT41603-100	The Native Antigen Company	0.1	EUR 395.28
Description: Bacteria are cultured on solid medium, harvested, washed and solubilised. The antigen is partially purified by detergent extraction and centrifugation.

Helicobacter Pylori Antigen
NAT41603-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Bacteria are cultured on solid medium, harvested, washed and solubilised. The antigen is partially purified by detergent extraction and centrifugation.

Campylobacter Jejuni Antigen
NAT41600-100	The Native Antigen Company	0.1	EUR 395.28
Description: Campylobacter jejuni is cultured on solid medium. This antigen is a partially purified detergent extract of the membrane fraction. The main component is an outer membrane protein, approximately 45 kDa in SDS-PAGE.

Campylobacter Jejuni Antigen
NAT41600-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Campylobacter jejuni is cultured on solid medium. This antigen is a partially purified detergent extract of the membrane fraction. The main component is an outer membrane protein, approximately 45 kDa in SDS-PAGE.

Chlamydia Trachomatis Antigen
CT022-1000	The Native Antigen Company	1.0	EUR 382.41
Description: Chlamydia trachomatis antigens from clarified cell lysate.

Mycoplasma Pneumoniae Antigen
MP901-1000	The Native Antigen Company	1.0	EUR 1019.75
Description: Mycoplasma pneumoniae whole organisms that have been purified and detergent treated to enrich antigenic proteins and decrease background.

Mycoplasma Pneumoniae Antigen
MP901-200	The Native Antigen Company	0.2	EUR 272.96
Description: Mycoplasma pneumoniae whole organisms that have been purified and detergent treated to enrich antigenic proteins and decrease background.

Neisseria Gonorrhoeae Antigen
NG01-1000	The Native Antigen Company	1.0	EUR 382.41
Description: Homogenised lysate of pure bacteria.

Echovirus Antigen, Recombinant
REC31776-100	The Native Antigen Company	0.1	EUR 279.4
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus

Echovirus Antigen, Recombinant
REC31776-500	The Native Antigen Company	0.5	EUR 1053.23
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus

Trichomonas Vaginalis Antigen
TV01-1000	The Native Antigen Company	1.0	EUR 424.9
Description: Washed & lysed suspension of whole protozoa

Echinococcus Granulosus Antigen
NAT41602-100	The Native Antigen Company	0.1	EUR 395.28
Description: Echinococcus granulosus material is derived from bovine hydatid cyst as sterile filtered cyst fluid.

Echinococcus Granulosus Antigen
NAT41602-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Echinococcus granulosus material is derived from bovine hydatid cyst as sterile filtered cyst fluid.

Enterovirus Antigen, Recombinant
REC31772-100	The Native Antigen Company	0.1	EUR 279.4
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus IgA, IgG and IgM ELISA.

Enterovirus Antigen, Recombinant
REC31772-500	The Native Antigen Company	0.5	EUR 1053.23
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus IgA, IgG and IgM ELISA.

Cytomegalovirus Purified Antigen
CMV-HP-100	The Native Antigen Company	0.1	EUR 321.89
Description: A concentrated source of human Cytomegalovirus particles purified by sucrose gradient ultracentrifugation.

Cytomegalovirus Purified Antigen
CMV-HP-1000	The Native Antigen Company	1.0	EUR 2115.47
Description: A concentrated source of human Cytomegalovirus particles purified by sucrose gradient ultracentrifugation.

Cytomegalovirus Purified Antigen
CMV-HP-500	The Native Antigen Company	0.5	EUR 1291.43
Description: A concentrated source of human Cytomegalovirus particles purified by sucrose gradient ultracentrifugation.

Astrovirus Antigen (Type 1)
AS02-1000	The Native Antigen Company	1.0	EUR 1669.97
Description: A concentrated source of Astrovirus particles and Astrovirus proteins from a lysate of host cells.

Astrovirus Antigen (Type 1)
AS02-200	The Native Antigen Company	0.2	EUR 446.78
Description: A concentrated source of Astrovirus particles and Astrovirus proteins from a lysate of host cells.

Yersinia pestis V antigen
REC32028-100	The Native Antigen Company	0.1	EUR 326.34
Description: Yersinia pestis V antigen (LcrV antigen, capsule protein fraction 1, type III secretion system needle tip protein). The protein is produced as an N-terminal GST fusion, with GST being cleaved by thrombin and is not present in the final product.

Yersinia pestis V antigen
REC32028-500	The Native Antigen Company	0.5	EUR 1386.95
Description: Yersinia pestis V antigen (LcrV antigen, capsule protein fraction 1, type III secretion system needle tip protein). The protein is produced as an N-terminal GST fusion, with GST being cleaved by thrombin and is not present in the final product.

Canine Herpes Virus Antigen
NAT41592-100	The Native Antigen Company	0.1	EUR 395.28
Description: Canine Herpes virus, produced in MDCK cells.

mycobacterium tuberculosis Antigen 85A Recombinant Protein
PROTP9WQP3	BosterBio	10ug	EUR 265
Description: mycobacterium tuberculosis Antigen 85A Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 44-338aa. Application(s): SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Canine Adenovirus 1 Antigen
NAT41587-100	The Native Antigen Company	0.1	EUR 395.28
Description: Canine Adenovirus Type 1, produced in A-72 cells.

Canine Adenovirus 2 Antigen
NAT41588-100	The Native Antigen Company	0.1	EUR 395.28
Description: Canine Adenovirus Type 2, produced in A-72 cells.

Toxoplasma gondii IgM antigen
TG-IgM-100	The Native Antigen Company	0.1mg	EUR 139.86
Description: A highly purified source of Toxoplasma gondii membrane antigens, optimised for IgM detection.

Toxoplasma gondii IgM antigen
TG-IgM-1000	The Native Antigen Company	1.0mg	EUR 1189.51
Description: A highly purified source of Toxoplasma gondii membrane antigens, optimised for IgM detection.

Canine Distemper Virus Antigen
NAT41591-100	The Native Antigen Company	0.1	EUR 395.28
Description: Canine Distemper virus, produced in SL-29 cells.

Canine Parainfluenza Virus Antigen
NAT41593-100	The Native Antigen Company	0.1	EUR 395.28
Description: Canine Parainfluenza virus antigen, produced in MDCK cells.

Feline Panleukopenia Virus Antigen
NAT41597-100	The Native Antigen Company	0.1	EUR 395.28
Description: Feline Panleukopenia Virus (FPLV; feline parvovirus) antigen, produced in CRFK cells.

Norovirus GII.4 VP1 Antigen
REC31620-100	The Native Antigen Company	0.1	EUR 305.15
Description: Recombinant Norovirus GII.4 VP1 antigen produced in 293 human cells.

Norovirus GII.4 VP1 Antigen
REC31620-1000	The Native Antigen Company	1.0	EUR 1847.65
Description: Recombinant Norovirus GII.4 VP1 antigen produced in 293 human cells.

Norovirus GII.4 VP1 Antigen
REC31620-500	The Native Antigen Company	0.5	EUR 1076.4
Description: Recombinant Norovirus GII.4 VP1 antigen produced in 293 human cells.

Cytomegalovirus Cell Lysate Antigen
CMV-CL-100	The Native Antigen Company	0.1	EUR 225.32
Description: A clarified cell lysate containing a mix of CMV antigens from all stages of the replication life cycle.

Cytomegalovirus Cell Lysate Antigen
CMV-CL-1000	The Native Antigen Company	1.0	EUR 1435.63
Description: A clarified cell lysate containing a mix of CMV antigens from all stages of the replication life cycle.

Feline Viral Rhinotracheitis Antigen
NAT41598-100	The Native Antigen Company	0.1	EUR 395.28
Description: Feline Viral Rhinotracheitis (FVR; feline herpes virus, FHV-1) antigen, produced in CRFK cells.

Herpes Simplex Virus 1 Antigen
HS105-1000	The Native Antigen Company	1.0	EUR 1189.71
Description: HSV-1 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Herpes Simplex Virus 1 Antigen
HS105-200	The Native Antigen Company	0.2	EUR 297.43
Description: HSV-1 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Herpes Simplex Virus 2 Antigen
HS205-1000	The Native Antigen Company	1.0	EUR 1189.71
Description: HSV-2 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Herpes Simplex Virus 2 Antigen
HS205-200	The Native Antigen Company	0.2	EUR 297.43
Description: HSV-2 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Ross River Virus Recombinant Antigen
REC31969-100	The Native Antigen Company	0.1	EUR 764.81
Description: Recombinant Ross River virus antigen expressed in HEK293 cells as virus-like particles and purified by sucrose density gradients and precipitation. Virus-like particles were then solubilized in PBS containing 0.2% SDS to prevent aggregation of particles. The preparation is not particulate. Antigens in the preparation include Envelope proteins 1 and 2 and Nucleoprotein.

Ross River Virus Recombinant Antigen
REC31969-500	The Native Antigen Company	0.5	EUR 2871.26
Description: Recombinant Ross River virus antigen expressed in HEK293 cells as virus-like particles and purified by sucrose density gradients and precipitation. Virus-like particles were then solubilized in PBS containing 0.2% SDS to prevent aggregation of particles. The preparation is not particulate. Antigens in the preparation include Envelope proteins 1 and 2 and Nucleoprotein.

Canine Adenovirus 1 and 2 Antigen
NAT41589-100	The Native Antigen Company	0.1	EUR 395.28
Description: A mixture of Canine Adenovirus Type 1 and Type 2, produced in A-72 cells.

Hepatitis B Virus E Antigen (HBeAg)
REC31677-100	The Native Antigen Company	0.1	EUR 460.95
Description: Recombinant Hepititis B Virus E Protein (HBeAg).

Hepatitis B Virus E Antigen (HBeAg)
REC31677-500	The Native Antigen Company	0.5	EUR 1382.84
Description: Recombinant Hepititis B Virus E Protein (HBeAg).

Hepatitis B Virus Core Antigen (HBcAg)
REC31689-100	The Native Antigen Company	0.1	EUR 460.95
Description: Recombinant Hepititis B Virus Core Protein (HBcAg).

Hepatitis B Virus Core Antigen (HBcAg)
REC31689-500	The Native Antigen Company	0.5	EUR 1382.84
Description: Recombinant Hepititis B Virus Core Protein (HBcAg).

Hepatitis C Virus Core Antigen (HCcAg)
REC31693-100	The Native Antigen Company	0.1	EUR 489.27
Description: Recombinant Hepatitis C Virus core protein is produced in E. coli and fused with a His-tag.

Hepatitis C Virus Core Antigen (HCcAg)
REC31693-500	The Native Antigen Company	0.5	EUR 1469.11
Description: Recombinant Hepatitis C Virus core protein is produced in E. coli and fused with a His-tag.

Feline Infectious Peritonitis Virus Antigen
NAT41596-100	The Native Antigen Company	0.1	EUR 395.28
Description: Feline Infectious Peritonitis virus (FIPV; feline coronavirus) antigen, produced in CRFK cells.

Herpes Simplex Virus 1 Lysate Antigen
HS104-1000	The Native Antigen Company	1.0	EUR 272.96
Description: A high concentration of HSV-1 particles and proteins from a clarified cell lysate.

Herpes Simplex Virus 2 Lysate Antigen
HS204-1000	The Native Antigen Company	1.0	EUR 272.96
Description: A high concentration of HSV-2 particles and proteins from a clarified cell lysate.

Toxoplasma gondii IgG antigen, RH strain
TG-IgG-1000	The Native Antigen Company	1.0 mg	EUR 338
Description: A concentrated source of Toxoplasma gondii tachyzoite antigens, optimised for IgG detection, diluted to 0.5 mg/ml.

Toxoplasma gondii IgG antigen, RH strain
TG-IgG-200	The Native Antigen Company	0.2 mg	EUR 174.83
Description: A concentrated source of Toxoplasma gondii tachyzoite antigens, optimised for IgG detection, diluted to 0.5 mg/ml.

Toxoplasma gondii IgG antigen, RH strain
TG-IgG-5000	The Native Antigen Company	5.0 mg	EUR 1436.48
Description: A concentrated source of Toxoplasma gondii tachyzoite antigens, optimised for IgG detection, diluted to 0.5 mg/ml.

Legionella pneumophila antigen (native extract)
NAT41580-100	The Native Antigen Company	0.1	EUR 395.28
Description: Inactivated Legionella pneumophila antigen from culture.

Streptococcus Pneumoniae Antigen, Native Extract
NAT41604-100	The Native Antigen Company	0.1	EUR 395.28
Description: Inactivated Streptococcus pneumoniae antigen from culture.

Leptospira Biflexa Antigen (Strain Patoc 1)
NAT41582-100	The Native Antigen Company	0.1	EUR 395.28
Description: Inactivated Leptospira biflexa antigen (Strain Patoc 1) from broth culture.

Leptospira Biflexa Antigen (Strain Patoc 1)
NAT41582-500	The Native Antigen Company	0.5	EUR 1187.13
Description: Inactivated Leptospira biflexa antigen (Strain Patoc 1) from broth culture.

Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus
MBS204142-001mg	MyBiosource	0.01mg	EUR 230

Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus
MBS204142-005mg	MyBiosource	0.05mg	EUR 405

Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus
MBS204142-025mg	MyBiosource	0.25mg	EUR 985

Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus
MBS204142-5x025mg	MyBiosource	5x0.25mg	EUR 4180

Mouse Anti-Hepatitis B Surface Antigen Antibody (1834)
MAB12230-100	The Native Antigen Company	0.1	EUR 278.11
Description: Mouse monoclonal antibody specific for Hepatitis B virus surface antigen (1834).

Mouse Anti-Hepatitis B Surface Antigen Antibody (1837)
MAB12231-100	The Native Antigen Company	0.1	EUR 278.11
Description: Mouse monoclonal antibody specific for Hepatitis B virus surface antigen (1837)

Mouse Anti-Hepatitis B Surface Antigen Antibody (1838)
MAB12232-100	The Native Antigen Company	0.1	EUR 278.11
Description: Mouse monoclonal antibody specific for Hepatitis B virus surface antigen (1838)

Mouse Anti-Hepatitis B Virus E Antigen Antibody (1892)
MAB12226-100	The Native Antigen Company	0.1	EUR 198.28
Description: Mouse monoclonal antibody specific for Hepatitis B virus e antigen (1892)

Mouse Anti-Hepatitis B Virus E Antigen Antibody (1893)
MAB12227-100	The Native Antigen Company	0.1	EUR 278.11
Description: Mouse monoclonal antibody specific for Hepatitis B virus e antigen (1893)

Mouse Anti-Hepatitis B Virus E Antigen Antibody (1894)
MAB12228-100	The Native Antigen Company	0.1	EUR 278.11
Description: Mouse monoclonal antibody specific for Hepatitis B virus e antigen (1894)

Mouse Anti-Hepatitis B Virus E Antigen Antibody (M132)
MAB12298-100	The Native Antigen Company	0.1	EUR 279.4
Description: Mouse monoclonal antibody specific for Hepatitis B virus e antigen (M132)

Mouse Anti-Hepatitis B Virus E Antigen Antibody (M132)
MAB12298-500	The Native Antigen Company	0.5	EUR 701.72
Description: Mouse monoclonal antibody specific for Hepatitis B virus e antigen (M132)

Mouse Anti-Hepatitis B Virus E Antigen Antibody (M133)
MAB12299-100	The Native Antigen Company	0.1	EUR 279.4
Description: Mouse monoclonal antibody specific for Hepatitis B virus e antigen (M133)

Mouse Anti-Hepatitis B Virus E Antigen Antibody (M133)
MAB12299-500	The Native Antigen Company	0.5	EUR 701.72
Description: Mouse monoclonal antibody specific for Hepatitis B virus e antigen (M133)

Mycobacterium Tuberculosis major secretory protein Antigen 85A Protein
abx262511-10mg	Abbexa	10 mg	EUR 325

Mycobacterium Tuberculosis major secretory protein Antigen 85A Protein
20-abx262511	Abbexa	Ask for price Ask for price Ask for price	2 µg 10 ug 1 mg

Mycobacterium Tuberculosis major secretory protein Antigen 85A Protein
abx262511-25mg	Abbexa	25 mg	EUR 6575

Mycobacterium Tuberculosis major secretory protein Antigen 85A Protein
abx262511-5mg	Abbexa	5 mg	EUR 225

Recombinant Mycobacterium Tuberculosis major secretory protein Antigen 85A
MBS144787-0002mg	MyBiosource	0.002mg	EUR 240

Recombinant Mycobacterium Tuberculosis major secretory protein Antigen 85A
MBS144787-001mg	MyBiosource	0.01mg	EUR 310

Recombinant Mycobacterium Tuberculosis major secretory protein Antigen 85A
MBS144787-1mg	MyBiosource	1mg	EUR 5345

Recombinant Mycobacterium Tuberculosis major secretory protein Antigen 85A
MBS144787-5x1mg	MyBiosource	5x1mg	EUR 23725

Mycobacterium Tuberculosis major secretory protein Antigen 85A Recombinant
rAP-3658	Angio Proteomie	Inquiry	Ask for price

Yersinia pestis chain B F1 capsule antigen, C-terminal His-tag
REC32027-100	The Native Antigen Company	0.1	EUR 326.34
Description: Yersinia pestis chain B F1 capsule antigen (Antig_Caf1)

Yersinia pestis chain B F1 capsule antigen, C-terminal His-tag
REC32027-500	The Native Antigen Company	0.5	EUR 1386.95
Description: Yersinia pestis chain B F1 capsule antigen (Antig_Caf1)

Ag85A Mycobacterium Tuberculosis major secretory protein Antigen 85A Recombinant Protein
PROTP0A4V2	BosterBio	Regular: 10ug	EUR 250
Description: Recombinant Mycobacterium tuberculosis Ag85A (43-338 a.a) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a.a in total). Antigen 85A is fused to a 6 amino acid His tag at C-terminus and purified by conventional chromatographic techniques.

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A)
MBS6006525-02mL	MyBiosource	0.2(mL	EUR 695

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A)
MBS6006525-5x02mL	MyBiosource	5x0.2mL	EUR 2975

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (AP)
MBS6301005-02mL	MyBiosource	0.2mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (AP)
MBS6301005-5x02mL	MyBiosource	5x0.2mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (PE)
MBS6301015-02mL	MyBiosource	0.2mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (PE)
MBS6301015-5x02mL	MyBiosource	5x0.2mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (APC)
MBS6301006-02mL	MyBiosource	0.2mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (APC)
MBS6301006-5x02mL	MyBiosource	5x0.2mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (FITC)
MBS6301008-02mL	MyBiosource	0.2mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (FITC)
MBS6301008-5x02mL	MyBiosource	5x0.2mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (Biotin)
MBS6301007-02mL	MyBiosource	0.2mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (Biotin)
MBS6301007-5x02mL	MyBiosource	5x0.2mL	EUR 4250

HCV NS3 1359-1456aa antigen
00154-V-01mg	Virogen	0,1 mg	EUR 321
Description: HCV NS3 1359-1456aa antigen. The protein contains immunodominant regions (1359-1456aa). available genotypes: 1a, 1b, 2b, 3, 4, 5, 6

HCV NS3 1359-1456aa antigen
00154-V-1000ug	Virogen	1000 ug	EUR 1539
Description: HCV NS3 1359-1456aa antigen. The protein contains immunodominant regions (1359-1456aa). available genotypes: 1a, 1b, 2b, 3, 4, 5, 6

HLA Class 1 Antigen A25,A32
MBS602885-01mL	MyBiosource	0.1mL	EUR 1290

HLA Class 1 Antigen A25,A32
MBS602885-5x01mL	MyBiosource	5x0.1mL	EUR 5650

HLA Class 1 Antigen A23,A24
MBS602995-01mL	MyBiosource	0.1mL	EUR 1380

HLA Class 1 Antigen A23,A24
MBS602995-5x01mL	MyBiosource	5x0.1mL	EUR 6060

A549 HCP Control Antigen
F237	Cygnus Technologies	50 ul	EUR 308.4
Description: A549 HCP Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

Cancer Antigen 242 Antigen
VAng-Cr3876-10kU	Creative Biolabs	10 kU	EUR 505.2
Description: Cancer Antigen 242 Antigen, Host/Source: Metastatic Liver. The purity is detected by gel filtration.

HLA Class 1 Antigen A29
MBS603635-01mL	MyBiosource	0.1mL	EUR 1380

HLA Class 1 Antigen A29
MBS603635-5x01mL	MyBiosource	5x0.1mL	EUR 6060

HLA Class 1 Antigen A11
MBS601315-01mL	MyBiosource	0.1mL	EUR 1380

HLA Class 1 Antigen A11
MBS601315-5x01mL	MyBiosource	5x0.1mL	EUR 6060

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 405)
MBS6301010-01mL	MyBiosource	0.1mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 405)
MBS6301010-5x01mL	MyBiosource	5x0.1mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 490)
MBS6301011-01mL	MyBiosource	0.1mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 490)
MBS6301011-5x01mL	MyBiosource	5x0.1mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 550)
MBS6301012-01mL	MyBiosource	0.1mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 550)
MBS6301012-5x01mL	MyBiosource	5x0.1mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 650)
MBS6301013-01mL	MyBiosource	0.1mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 650)
MBS6301013-5x01mL	MyBiosource	5x0.1mL	EUR 4250

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 750)
MBS6301014-01mL	MyBiosource	0.1mL	EUR 980

GAGE13, NT (GAGE13, GAGE12A, G antigen 13, G antigen 12A) (MaxLight 750)
MBS6301014-5x01mL	MyBiosource	5x0.1mL	EUR 4250

Antigen 85-A Antibody (HRP)
20-abx109227	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Antigen 85-A (fbpA) Antibody
abx110661-100l	Abbexa	100 µl	EUR 162.5

Antigen 85-A Antibody (FITC)
20-abx107809	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

A549 HCP Antigen Concentrate
F233G	Cusabio	200 ul	EUR 540
Description: A549 HCP Antigen Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Antigen 85-A (Ag85A) Antibody
20-abx110661	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

CEA Antigen (Antigen Grade)
GWB-378447	GenWay Biotech	1 mg	Ask for price

Cancer Antigen 50 Antigen
VAng-Cr3877-50kU	Creative Biolabs	50 kU	EUR 6642
Description: Cancer Antigen 50 Antigen, Host/Source: Human Cell Culture. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.

Here we describe strategies for the development of such fluorescent reporter M. tuberculosis strains, and their use together with confocal microscopy and movement cytometry approaches for single bacterium-level analyses of M. tuberculosis physiology and M. tuberculosis-host interactions.